First international external quality assessment of molecular diagnostics for Mers-CoV
Identifieur interne : 001790 ( Main/Exploration ); précédent : 001789; suivant : 001791First international external quality assessment of molecular diagnostics for Mers-CoV
Auteurs : Suzan D. Pas [Pays-Bas] ; Pranav Patel [Allemagne] ; Chantal Reusken [Pays-Bas] ; Cristina Domingo [Allemagne] ; Victor M. Corman [Allemagne] ; Christian Drosten [Allemagne] ; Ronald Dijkman [Suisse] ; Volker Thiel [Suisse] ; Norbert Nowotny [Oman, Autriche] ; Marion P. G. Koopmans [Pays-Bas] ; Matthias Niedrig [Allemagne]Source :
- Journal of Clinical Virology [ 1386-6532 ] ; 2015.
Descripteurs français
- KwdFr :
- Charge virale, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Internationalité, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de diagnostic moléculaire (normes), Évaluation de la compétence des laboratoires.
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- normes : Techniques de diagnostic moléculaire.
- virologie : Infections à coronavirus.
- Charge virale, Humains, Internationalité, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Évaluation de la compétence des laboratoires.
English descriptors
- KwdEn :
- Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Humans, Internationality, Laboratory Proficiency Testing, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (standards), Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Viral Load.
- MESH :
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- standards : Molecular Diagnostic Techniques.
- virology : Coronavirus Infections.
- Humans, Internationality, Laboratory Proficiency Testing, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Viral Load.
Abstract
We describe results of the first HCoV-MERS external quality control panel. 85% of the 189 returned results had a 100% score. 8.1% of laboratories produced false positive results. ORF1b target is not recommended for screenings PCR.
Url:
DOI: 10.1016/j.jcv.2015.05.022
PubMed: 26209385
PubMed Central: 7106520
Affiliations:
- Allemagne, Autriche, Oman, Pays-Bas, Suisse
- Berlin, District de Cologne, Hollande-Méridionale, Rhénanie-du-Nord-Westphalie, Vienne (Autriche)
- Berlin, Bonn, Rotterdam, Vienne (Autriche)
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Le document en format XML
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Pas</name><affiliation wicri:level="3"><nlm:aff id="aff0005">Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Patel, Pranav" sort="Patel, Pranav" uniqKey="Patel P" first="Pranav" last="Patel">Pranav Patel</name><affiliation wicri:level="3"><nlm:aff id="aff0010">Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation wicri:level="3"><nlm:aff id="aff0005">Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Domingo, Cristina" sort="Domingo, Cristina" uniqKey="Domingo C" first="Cristina" last="Domingo">Cristina Domingo</name><affiliation wicri:level="3"><nlm:aff id="aff0010">Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M." last="Corman">Victor M. Corman</name><affiliation wicri:level="3"><nlm:aff id="aff0015">Institute for virology, University of Bonn, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for virology, University of Bonn, Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff0020">German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="3"><nlm:aff id="aff0015">Institute for virology, University of Bonn, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for virology, University of Bonn, Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff0020">German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Dijkman, Ronald" sort="Dijkman, Ronald" uniqKey="Dijkman R" first="Ronald" last="Dijkman">Ronald Dijkman</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Federal Institute of Virology and Immunology, Berne and Mittelhäusern, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Federal Institute of Virology and Immunology, Berne and Mittelhäusern</wicri:regionArea><wicri:noRegion>Berne and Mittelhäusern</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0030">Vetsuisse Faculty, University of Berne, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Vetsuisse Faculty, University of Berne</wicri:regionArea><wicri:noRegion>University of Berne</wicri:noRegion></affiliation></author><author><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Federal Institute of Virology and Immunology, Berne and Mittelhäusern, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Federal Institute of Virology and Immunology, Berne and Mittelhäusern</wicri:regionArea><wicri:noRegion>Berne and Mittelhäusern</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0030">Vetsuisse Faculty, University of Berne, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Vetsuisse Faculty, University of Berne</wicri:regionArea><wicri:noRegion>University of Berne</wicri:noRegion></affiliation></author><author><name sortKey="Nowotny, Norbert" sort="Nowotny, Norbert" uniqKey="Nowotny N" first="Norbert" last="Nowotny">Norbert Nowotny</name><affiliation wicri:level="1"><nlm:aff id="aff0035">Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman</nlm:aff><country xml:lang="fr">Oman</country><wicri:regionArea>Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat</wicri:regionArea><wicri:noRegion>Muscat</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff0040">Institute of Virology, University of Veterinary Medicine Vienna, Vienna, Austria</nlm:aff><country xml:lang="fr">Autriche</country><wicri:regionArea>Institute of Virology, University of Veterinary Medicine Vienna, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Koopmans, Marion P G" sort="Koopmans, Marion P G" uniqKey="Koopmans M" first="Marion P. G." last="Koopmans">Marion P. G. Koopmans</name><affiliation wicri:level="3"><nlm:aff id="aff0005">Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Niedrig, Matthias" sort="Niedrig, Matthias" uniqKey="Niedrig M" first="Matthias" last="Niedrig">Matthias Niedrig</name><affiliation wicri:level="3"><nlm:aff id="aff0010">Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of Clinical Virology</title><idno type="ISSN">1386-6532</idno><idno type="eISSN">1873-5967</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Humans</term><term>Internationality</term><term>Laboratory Proficiency Testing</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Molecular Diagnostic Techniques (standards)</term><term>Real-Time Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Viral Load</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Charge virale</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Internationalité</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire (normes)</term><term>Évaluation de la compétence des laboratoires</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Techniques de diagnostic moléculaire</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Molecular Diagnostic Techniques</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Internationality</term><term>Laboratory Proficiency Testing</term><term>Real-Time Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Viral Load</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Charge virale</term><term>Humains</term><term>Internationalité</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Évaluation de la compétence des laboratoires</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">We describe results of the first HCoV-MERS external quality control panel.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">85% of the 189 returned results had a 100% score.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">8.1% of laboratories produced false positive results.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">ORF1b target is not recommended for screenings PCR.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, X" uniqKey="Lu X">X. Lu</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Pereyaslov, D" uniqKey="Pereyaslov D">D. Pereyaslov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K. Pyrc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dijkman, R" uniqKey="Dijkman R">R. Dijkman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kapoor, M" uniqKey="Kapoor M">M. Kapoor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kraaij Dirkzwager, M" uniqKey="Kraaij Dirkzwager M">M. Kraaij-Dirkzwager</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li><li>Autriche</li><li>Oman</li><li>Pays-Bas</li><li>Suisse</li></country><region><li>Berlin</li><li>District de Cologne</li><li>Hollande-Méridionale</li><li>Rhénanie-du-Nord-Westphalie</li><li>Vienne (Autriche)</li></region><settlement><li>Berlin</li><li>Bonn</li><li>Rotterdam</li><li>Vienne (Autriche)</li></settlement></list><tree><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Pas, Suzan D" sort="Pas, Suzan D" uniqKey="Pas S" first="Suzan D." last="Pas">Suzan D. Pas</name></region><name sortKey="Koopmans, Marion P G" sort="Koopmans, Marion P G" uniqKey="Koopmans M" first="Marion P. G." last="Koopmans">Marion P. G. Koopmans</name><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name></country><country name="Allemagne"><region name="Berlin"><name sortKey="Patel, Pranav" sort="Patel, Pranav" uniqKey="Patel P" first="Pranav" last="Patel">Pranav Patel</name></region><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M." last="Corman">Victor M. Corman</name><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M." last="Corman">Victor M. Corman</name><name sortKey="Domingo, Cristina" sort="Domingo, Cristina" uniqKey="Domingo C" first="Cristina" last="Domingo">Cristina Domingo</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Niedrig, Matthias" sort="Niedrig, Matthias" uniqKey="Niedrig M" first="Matthias" last="Niedrig">Matthias Niedrig</name></country><country name="Suisse"><noRegion><name sortKey="Dijkman, Ronald" sort="Dijkman, Ronald" uniqKey="Dijkman R" first="Ronald" last="Dijkman">Ronald Dijkman</name></noRegion><name sortKey="Dijkman, Ronald" sort="Dijkman, Ronald" uniqKey="Dijkman R" first="Ronald" last="Dijkman">Ronald Dijkman</name><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name></country><country name="Oman"><noRegion><name sortKey="Nowotny, Norbert" sort="Nowotny, Norbert" uniqKey="Nowotny N" first="Norbert" last="Nowotny">Norbert Nowotny</name></noRegion></country><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Nowotny, Norbert" sort="Nowotny, Norbert" uniqKey="Nowotny N" first="Norbert" last="Nowotny">Norbert Nowotny</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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